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The image was taken using an Oxalis triangularis (Purple Shamrock) plant. Removing one of the shamrock leaves and examining under my home epifluorescence microscope allowed me to view the different structures via light microscopy as well as via autofluorescence. Using an Hg (Mercury) bulb attachment and a filter cube with a UV excitation/emission, I was able to view the leaf's trichomes along its edges without processing using any staining or manipulations. The trichomes appeared to glow brightly off the edges. Taking a series of images and compiling them into a stacked image to obtain a completely in-focus trichome shows the detail and simplicity of the plant.
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Jeff Carpenter
Category
New York Photography - People
Country / Region:
United States
Photographer
Dancho Atanasov
Category
Architecture Photography - Building
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Bulgaria
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Elizabeth Storms
Category
Black & White Photography - Fine Art
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Canada
Photographer
Hungyi Chang
Category
Editorial Photography - Religious
Country / Region:
Taiwan